S-adenosysl-L-methionine:(Iso)eugenol O-methyltransferase, IEMT

26% PEG 4000, 0.3M magnesium nitrate,3% ethylene glycol, and 2 mM DTT

, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K

THE IDENTITY OF THE LIGAND ON CHAINS B-D IS MOST LIKELY EUG. THE AUTHOR INDICATES THAT THE 2FO-FC ELECTRON DENSITY MAP IS NOT VERY CLEAR AND THAT THE LIGAND WAS BUILT USING AN OMIT MAP. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

(Iso)eugenol O-methyltransferase (E.C.2.1.1.146)

directed evolution, SATURATION MUTAGENESIS, regioselectivity, TRANSFERASE