Mos1 transposase paired-end complex with left transposon end

Structural Basis for the Inverted Repeat Preferences of mariner Transposases.

100 mM Sodium Citrate pH 5.8, 100 mM Ammonium Acetate, 450 mM KCl, and 5 mM MnCl2, hanging drop, temperature 290K, VAPOR DIFFUSION, HANGING DROP

AUTHOR STATES THAT ENTRY SEQUENCE OF 4R79 IS CORRECT FOR MOS1. THE REFERENCE J7JQ07 ACTUALLY REFERS TO THE PEACH ELEMENT RATHER THAN MOS1. THE ENTRY SEQUENCE HAS 4 DIFFERENCES AGAINST J7JQ07, AS INDICATED IN FIGURE 2 OF THE PAPER BY MEDHORA ET AL IN GENETICS 128:311-318 JUNE,1991. PMCID:PMC1204469. THESE 4 DIFFERENCES SEEM TO MATCH WITH THE VARIANTS HIGHLIGHTED.

transposase, protein-DNA complex, transpososome, Rnase-H like catalytic fold Helix-turn-helix, DNA transposition, DNA cleavage, DNA integration, transposon, inverted repeats, Recombination-DNA complex