mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 3

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Around she goes: the structure of mouse Interleukin-12 p80

Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis.

PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)

Residues [1-22] encode a secretion signal which is should have been co-translationally removed.

IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40

BCS screen, 0.2M sodium formate, 0.1M sodium phosphate pH 6.0, 17% PEG Smear Medium, 10% Glycerol. Cryoprotected with up to 27.5% PEG Smear Medium prior to vitrification.

chain A and (resid 23:109 or resid 350:355)

chain B and (resid 23:109 or resid 350:355)

chain C and (resid 23:109 or resid 350:355)

chain D and (resid 23:109 or resid 350:355)

Homodimer, also present in PDB 6sff, 6smc, 6sp3 and 7pur.

HOMODIMER, ANTAGONIST, FIBRONECTIN, SECRETED GLYCOPROTEIN, CYTOKINE