Crystal structure of the ternary complex of sp-ASADH with NADP and 1,2,3-Benzenetricarboxylic acid

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

A cautionary tale of structure-guided inhibitor development against an essential enzyme in the aspartate-biosynthetic pathway.

double crystal monochromator and K-B pair of biomorph mirrors

ASA dehydrogenase, ASADH, Aspartate-beta-semialdehyde dehydrogenase

15-22% PEG 3350, 0.1 M sodium acetate, 0.1 M MES (pH 6.5), 3% Ethylene glycol, 10 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 295K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Aspartate-semialdehyde dehydrogenase (E.C.1.2.1.11)

protein-nadp-ligand complex, Dehydrogenase, Oxidoreductase, NADP binding