Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn/Fluoroacetate

Mapping the reaction coordinates of enzymatic defluorination.

15-24% PEG3350, 0.1-0.2M CaCl2 and 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

The Asp110Asn mutant in complex with fluoroacetate.

The Asp110Asn mutant in complex with chloroacetate.

The Asp110Asn mutant in complex with bromoacetate.

The Asp110Asn mutant/apo crystal structure

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: REFINED INDIVIDUALLY

Fluoroacetate dehalogenase (E.C.3.8.1.3)

FAcD, Defluorinase, Alpha/beta Hydrolase, HYDROLASE