Crystal structure of a eukaryotic group II intron lariat

RNA was prepared by in vitro transcription

0.4 mM spermine, 21% 2-Methyl-2,4-pentanediol, 175 mM magnesium acetate tetrahydrate, and 90 mM MES monohydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K

lariat, ribozyme, 2'-5' phosphodiester, RNA