CryoEM structure of bacterial transcription close complex (RPc)

Mechanisms of DNA opening revealed in AAA+ transcription complex structures.

20 mM Tris-HCl pH 8.0, 150 mM KCl, 10 mM MgCl2

Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex

The sample was mxied in vitro in an order to form complex

DNA-directed RNA polymerase subunit alpha

DNA-directed RNA polymerase subunit beta

DNA-directed RNA polymerase subunit beta'

DNA-directed RNA polymerase subunit omega

RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha

RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta

RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'

RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega

rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950

Biotechnology and Biological Sciences Research Council (BBSRC)

Medical Research Council (MRC, United Kingdom)

Cryo EM, RNA polymerase, transcription close complex, sigma54, TRANSCRIPTION