The Transcriptional Regulator PrfA-A218G mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif

A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.

Listeriolysin positive regulatory factor A

Listeriolysin positive regulatory protein,Listeriolysin regulatory protein,Listeriolysin transcriptional regulator PrfA,Pleitrophic regulatory factor A,Positive regulatory factor A,PrfA

Droplets of 4 microl protein-DNA solution (ratio 1:1.3) in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, and 1 mM EDTA at 6 mg per ml were mixed with 2 microl reservoir solution consisting of 8% PEG 8000, 100 mM sodium citrate pH 4.6, 100 mM Magnesium acetate and 20% glycerol.

Listeriolysin regulatory protein/DNA Complex

TRANSCRIPTION REGULATOR, ACTIVATION, LISTERIA MONOCYTOGENES, TRANSCRIPTION, VIRULENCE FACTOR, DNA BINDING PROTEIN