CRYSTAL STRUCTURE OF A PUTATIVE SUGAR PHOSPHATE ISOMERASE/EPIMERASE (AVA4194) FROM ANABAENA VARIABILIS ATCC 29413 AT 1.78 A RESOLUTION

Joint Center for Structural Genomics (JCSG)

Crystal structure of putative sugar phosphate isomerase/epimerase (YP_324688.1) from Anabaena variabilis ATCC 29413 at 1.78 A resolution

Single crystal Si(111) bent (horizontal focusing)

NANODROP, 0.2M CaCl2, 20.0% PEG 3350, No Buffer pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K

BIOMOLECULE: 1, 2, 3
SEE REMARK 350 FOR THE AUTHOR PROVIDED AND PROGRAM
GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION
ON BURIED SURFACE AREA.
THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC
UNIT WHICH CONSISTS OF 2 CHAINS. AUTHORS STATE THAT
THE EBI/PISA ANALYSIS SUPPORTS THE ASSIGNMENT OF A
TETRAMER AS A SIGNIFICANT OLIGOMERIZATION STATE.

SEQUENCE
THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG
MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE
LEAVING ONLY A GLYCINE (0) FOLLOWED BY THE TARGET SEQUENCE.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
2. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE
INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY
OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75
FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION.
3. RESIDUES 0-5 IN CHAIN A AND 0-6 IN CHAIN B ARE DISORDERED AND
NOT INCLUDED IN THE MODEL.
4. ZN IONS ARE MODELED BASED ON THE METAL EXCITATION SCAN.
5. A GLYCEROL MOLECULE FROM THE CRYO SOLUTION IS MODELED.
6. A LIGAND MOLECULE XYLOSE IS MODELED IN EACH MONOMER.

PUTATIVE SUGAR PHOSPHATE ISOMERASE/EPIMERASE, STRUCTURAL GENOMICS, JOINT CENTER FOR STRUCTURAL GENOMICS, JCSG, PROTEIN STRUCTURE INITIATIVE, PSI-2, ISOMERASE