Crystal structure of the biofilm forming subunit of the E. coli common pilus: donor strand complemented (DSC) EcpA

Structural insights into the biogenesis and biofilm formation by the Escherichia coli common pilus.

N-terminal truncated EcpA (residues 19-173) domain strand complimented with the N-terminus of EcpD (residues 1-17)
EcpA (residues 41-195) domain, N-terminus of EcpD (residues 21-37)

20% PEG 3350, 200 mM Na Citrate , pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Full length domain swapped dimer of EcpA

RESIDUES 178-191 CORRESPONDS TO RESIDUES 1-17 OF MATURE ECPD (UNIPROT ID: Q8CWD6).

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

pilin, Ig-like fold, biofilm, adhesion, Immunoglobulin-like fold, major pilin domain involved in biofilms, intermolecular and hydrophobic abiotic surface binding, extracellular membrane, fusion protein, Chimera protein, CELL ADHESION