SpCas9 bound to PTPRC off-target1 DNA substrate

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural basis for Cas9 off-target activity.

CRISPR-associated endonuclease Cas9/Csn1

chain 'B' and (resid 1089 through 1367 )

crispr, cas9, off-target, ternary complex, HYDROLASE