The apoenzyme structure of the yeast SR protein kinase, Sky1p

Nucleotide-Induced Conformational Changes in the Saccharomyces cerevisiae SR Protein Kinase, Sky1p, Revealed by X-Ray Crystallography

1.5M (NH4)2SO4, 100mM Na Acetate pH 4.5, 15% Ethylene Glycol. Crystals then dialyzed into 30% PEG 400 and 100mM Na Acetate pH 4.5, hanging drop, crystals soaked into low salt buffer, temperature 298.0K

SEQUENCE
137 amino acids truncated from N-terminus,
RESIDUES 305-538 WERE DELETED AND REPLACED WITH VAL-ASP