E. COLI (LACZ) BETA-GALACTOSIDASE (G794A) WITH IPTG BOUND

1-methylethyl 1-thio-beta-D-galactopyranoside
ISOPROPYL-1-BETA-D-THIOGALACTOSIDE; 1-(ISOPROPYLTHIO)-BETA-GALACTOPYRANSIDE; 1-methylethyl 1-thio-beta-D-galactoside; 1-methylethyl 1-thio-D-galactoside; 1-methylethyl 1-thio-galactoside

Structural Basis for the Altered Activity of Gly794 Variants of Escherichia coli Beta-Galactosidase

LEU B 685 HAS WRONG CHIRALITY AT ATOM CA

PEG 8000, NACL, MGCL2, DTT, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K

HETEROGEN

EACH HETEROATOM HAS BEEN ASSIGNED A CHAIN ID
AND RESIDUE NUMBER BASED ON AN EQUIVALENCY TO
HETEROATOMS IN PDB ENTRY 1DP0 (NATIVE
BETA-GALACTOSIDASE).

THE NUMBERING IS AS FOLLOWS:

2001-2002 LIGAND IPT,
ISOPROPYL-1-BETA-D-THIOGALACTOSIDE
3001-3999 IONS (MG++ AND NA+)
4001-7999 WATER MOLECULES
THOSE WATER MOLECULES WITHOUT AN EQUIVALENT
IN 1DP0 START AT 7500
8401-8799 DMS