NMR structure of the NALP1 Pyrin domain (PYD)

NMR structure of the apoptosis- and inflammation-related NALP1 pyrin domain

Residues 1-56 correspond to the fused Immunoglobulin G binding protein G (SWS P06654, residues 228-282)
NACHT-, LRR- and PYD-containing protein 2

Death effector filament-forming ced-4-like apoptosis protein, Nucleotide-binding domain and caspase recruitment domain, Caspase recruitment domain protein 7

SEQUENCE
Residues 1-58 are missing from the coordinates
because they were not included in the model.
Residues 57 and 58 are independent linking residues,
which have been inserted to provide distance and
flexibiltiy in this two-domain fusion protein.

Protein was expressed as a fusion protein with G B1 to enhance solubility (Zhou et al., J. Biomol. NMR 20, 11-14)

1mM NALP1 PYD U-15N,13C; 50mM Na / PO4 - Buffer; 50mM NaCl; 1mM CHAPS; 20mM DTT (D10); 0.02% NaN3; 0.1mM EDTA; protease inhibitor cocktail (Complete, Roche); 95% H2O, 5% D2O