PntC-AEPT: fusion protein of phosphonate-specific cytidylyltransferase and 2-aminoethylphosphonate (AEP) transaminase from Treponema denticola in complex with cytidine monophosphate-AEP

The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis.

Nucleotidyl transferase/aminotransferase, class V

Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)

0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 20% (w/v) polyethylene glycol 8,000

Natural Sciences and Engineering Research Council (NSERC, Canada)

Additionally confirmed via dynamic light scattering

Phosphonate, Cytidylyltransferase, Cytidine monophosphate- 2-aminoethylphosphonate (CMP-AEP), BIOSYNTHETIC PROTEIN