DNA-binding domain of a p53 homolog from the hydrothermal vent annelid Alvinella pompejana

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Evolutionary history of the p53 family DNA-binding domain: insights from an Alvinella pompejana homolog.

Protein solution: 7.2 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Crystallization buffer: 22% PEG 3350, 0.2M MgCl2, 0.1M Tris, pH 8.3. Drop size: 300 nL (150 + 150). Crystals were cryoprotected with mother liquor supplemented with 23% ethylene glycol.

p53 family, transcription factor, protein evolution, hydrothermal vents, extremophile, protein stability, TRANSCRIPTION