Crystal structure of the Na+-translocating NADH: ubiquinone oxidoreductase from Vibrio cholerae

Structure of the V. cholerae Na+-pumping NADH:quinone oxidoreductase.

His6-tag was cleaved off by HRV-3C protease.
Na(+)-translocating NADH-quinone reductase subunit A

Contains FMN covalently bound to Threonin 236.
Na(+)-translocating NADH-quinone reductase subunit B

Contains FMN covalently bound to Threonin 225.
Na(+)-translocating NADH-quinone reductase subunit C

Na(+)-translocating NADH-quinone reductase subunit D

Na(+)-translocating NADH-quinone reductase subunit E

Na(+)-translocating NADH-quinone reductase subunit F

Na(+)-translocating NQR subunit A,NQR complex subunit A,NQR-1 subunit A

Na(+)-translocating NQR subunit B,NQR complex subunit B,NQR-1 subunit B

Na(+)-translocating NQR subunit C,NQR complex subunit C,NQR-1 subunit C

Na(+)-translocating NQR subunit D,NQR complex subunit D,NQR-1 subunit D

Na(+)-translocating NQR subunit E,NQR complex subunit E,NQR-1 subunit E

Na(+)-translocating NQR subunit F,NQR complex subunit F,NQR-1 subunit F

25% PEG2000 MME, 0.2 M KSCN, 0.1 M Tris-acetate pH 8.5

Respiratory Complex, NADH:ubiquinone oxidoreductase, Sodium translocation, FAD, covalent FMN, Riboflavin, OXIDOREDUCTASE