Post-catalytic nicked complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and newly incorporated AMP

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

Unexpected behavior of DNA polymerase Mu opposite template 8-oxo-7,8-dihydro-2'-guanosine.

DNA (5'-D(*CP*GP*GP*CP*(8OG)P*TP*AP*CP*G)-3')

polydeoxyribonucleotide/polyribonucleotide hybrid

100mM HEPES pH 7.5, 100mM NaCl, 10% PEG4000

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

The authors state that anomalous signal is observed for the metal A site (peak greater than 10 sigma), though no anomalous scatterers were added to the crystallization or soaking solutions. Therefore, this metal has been modeled as manganese, based on the coordination geometry and interatomic distances.

tetramer consists of one protein molecule (chain A) bound to one DNA duplex comprised of three annealed DNA strands (chains T, P, and D)

DNA-directed DNA/RNA polymerase mu/DNA Complex

Family X polymerase, NHEJ, TRANSFERASE, TRANSFERASE-DNA complex