CRYSTAL STRUCTURE OF A PUTATIVE PHOSPHONOPYRUVATE HYDROLASE (MLL9387) FROM MESORHIZOBIUM LOTI MAFF303099 AT 2.15 A RESOLUTION

Joint Center for Structural Genomics (JCSG)

Crystal structure of hypothetical protein (NP_085906.1) from Mesorhizobium loti at 2.15 A resolution

1M LONG RH COATED BENT CYLINDRICAL MIRROR FOR HORIZONTAL AND VERTICAL FOCUSING

NANODROP, 3.0% PEG 4000, 23.3% Glycerol, 0.1M Sodium acetate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 4.00

SEQUENCE
THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG
MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV
PROTEASE LEAVING ONLY A GLYCINE, FOLLOWED BY THE
TARGET SEQUENCE.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY. 3. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75 FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION. 4. ACETATE, GLYCEROL AND CHLORIDE ATOMS, PRESENT IN THE CRYSTALLIZATION SOLUTION, ARE MODELED INTO THE STRUCTURE.

PUTATIVE PHOSPHONOPYRUVATE HYDROLASE, STRUCTURAL GENOMICS, JOINT CENTER FOR STRUCTURAL GENOMICS, JCSG, PROTEIN STRUCTURE INITIATIVE, PSI-2, HYDROLASE