Human 14-3-3 sigma in complex with human MDM2 peptide

S-CYSTEINESULFINIC ACID; S-SULFINOCYSTEINE

Characterizing the protein-protein interaction between MDM2 and 14-3-3 sigma ; proof of concept for small molecule stabilization.

TEV protease cleavable N-terminal His-tag. Cterm Delta-17 construct

I modified this in order to be able to submit, but please correct the sequence of the peptide: RRRAI(SEP)ETEENSDELSGERQRKRHK(SEP)DSISL

Epithelial cell marker protein 1,Stratifin

Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2

1 M HEPES pH 7.4 to 7.6, 0.19 M CaCl2, 30 to 31 % PEG 400, 5 % glycerol

Engineering and Physical Sciences Research Council

Biotechnology and Biological Sciences Research Council (BBSRC)

SIGNALING PROTEIN-PEPTIDE complex, PROTEIN BINDING