Leishmania major Tyrosyl-tRNA synthetase in complex with tyrosinol, triclinic crystal form 1

Medical Structural Genomics of Pathogenic Protozoa (MSGPP)

The Double-Length Tyrosyl-tRNA Synthetase from the Eukaryote Leishmania major Forms an Intrinsically Asymmetric Pseudo-Dimer.

1:1:0.5 native protein solution (14 mg/ml LmTyrRS in MSGPP buffer containing 1 mM TCEP and 10 mM tyrosinol): well solutionn (16% PEG 3350, 0.2 M potassium formate pH 7.5): seed stock (1:100 dilution in SGPP buffer); cryoprotected by quick soak in 15% ethylene glycol, 18.75% PEG 3350, 187.5 mM potassium formate pH 7.5, 10mM tyrosinol, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Medical Structural Genomics of Pathogenic Protozoa

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES RESIDUAL ONLY.

aminoacyl-tRNA synthetase, tRNA ligase, aaRS, TyrRS, pseudodimer, translation, ATP-binding, nucleotide-binding, ligase, Structural Genomics, Medical Structural Genomics of Pathogenic Protozoa, MSGPP