Crystal structure of Mus musculus (Mm) Endonuclease V in complex with a 23mer RNA oligo containing an inosine after a 10 min soak in 10 mM Mn2+

Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.

DNA/RNA (5'-R(P*CP*GP*GP*UP*AP*AP*CP*CP*C)-D(P*I)-R(P*AP*UP*AP*UP*GP*CP*AP*UP*GP*CP*AP*UP*U)-3')

polydeoxyribonucleotide/polyribonucleotide hybrid

0.2 M potassium sodium tartrate, 20-25% w/v PEG3350

Chains C and D are partially cleaved between residues 11 and 12. Conformation A represents the substrate state. Conformation B represents the product state.

Nucleic acid hydrolysis, RNA recognition, metal ion dependent catalysis, DNA damage, adenosine deamination, HYDROLASE-RNA complex