Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.

CRISPR-associated endonuclease Cas9/Csn1

polydeoxyribonucleotide/polyribonucleotide hybrid

Cas9, CRISPR, chRDNA, TRAC, nuclease, HYDROLASE