Complex of a C. perfringens O-GlcNAcase with a fragment hit

O-GlcNAcase Fragment Discovery with Fluorescence Polarimetry.

Beta-N-acetylglucosaminidase,Beta-N-acetylhexosaminidase,Beta-hexosaminidase,GH84C,Hexosaminidase B,N-acetyl-beta-D-glucosaminidase

Apo crystals were grown in 0.6 M NaAcetate pH 7.5, 0.175 M cadmium sulphate (ML). Crystals were then transferred into a drop supplemented with 10 mM ligand and incubated for 4 h. Crystals were cryo-protected by short immersion in ML supplemented with 20% glycerol and saturated with ligand.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

HYDROLASE, Fragment complex, CARBOHYDRATE