crystal structure of PDE10A2 in complex with AMP

From the Cover: Structural insight into substrate specificity of phosphodiesterase 10.

cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

The native PDE10A2 crystals were grown against a well buffer of 0.1 M HEPES (pH 7.5), 0.2 M MgCl2, 50 mM beta-mercaptoethanol(BME), and 16% PEG3350. The complex of PDE10A2-AMP was prepared by soaking the crystals in 20 mM cAMP, VAPOR DIFFUSION, HANGING DROP, temperature 277K

cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A (E.C.3.1.4.17)

two molecules in the crystallographic asymmetric unit may not be biologically relevant.