The crystal structure of Erwinia tasmaniensis levansucrase in complex with (S)-1,2,4-butanentriol

Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol.

The Structure of Sucrose-Soaked Levansucrase Crystals from Erwinia tasmaniensis reveals a Binding Pocket for Levanbiose.

Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora.

Levansucrase (Beta-D-fructofuranosyl transferase)

Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)
Escherichia coli 'BL21-Gold(DE3)pLysS AG'

PEG 3000 15%, 1,2,4-Butanetriol (CAS 3068-00-6) 20%, NDSB 256 1%, 2.5 mM Manganese(II) chloride tetrahydrate, 2.5 mM Cobalt(II) chloride hexahydrate, 2.5 mM Nickel(II) chloride hexahydrate, 2.5 mM Zinc acetate dihydrate)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

fructosyltransferase, levan, FOS, transfructosylation, fructo-oligosaccharides, oligofructans, TRANSFERASE