Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi

Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM.

Rosetta iterative refinement was combined with MDFF.

5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 9.0 %(v/v) DMSO

Filamentous jasplakinolide-stabilized alpha actin in complex with ADP-Pi

Images were binned twice during motion correction.

Manual backside blotting using Whatman filter paper No.5.

Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP

Cryo-EM structure of F-actin in complex with ADP

Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized)

Cryo-EM structure of phalloidin-stabilized F-actin (aged)

Cryo-EM structure of jasplakinolide-stabilized F-actin (aged)

Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized)

Cytoskeleton, nucleotide states, filament stability, cell migration, STRUCTURAL PROTEIN