Structure of active transcription elongation complex Pol II-DSIF-ELL2-EAF1(composite structure)

Allosteric transcription stimulation by RNA polymerase II super elongation complex.

This is a composite map of map 9 and 10. The particle number reported is the sum of particles from both reconstructions.

Transcription elongation complex of RNA polymerase II with elongation factors DSIF and ELL2, EAF1

DNA-directed RNA polymerase II subunit RPB1

DNA-directed RNA polymerase subunit beta

DNA-directed RNA polymerase II subunit RPB3

DNA-directed RNA polymerase II subunit E

DNA-directed RNA polymerase II subunit F

DNA-directed RNA polymerase II subunit RPB7

DNA-directed RNA polymerases I, II, and III subunit RPABC3

DNA-directed RNA polymerase II subunit RPB9

DNA-directed RNA polymerases I, II, and III subunit RPABC5

RNA polymerase II elongation factor ELL2

The nucleotide A47 in the structure was not in the original RNA scaffold (46-mer) and was extended by Pol II during complex formation in the presence of ATP.

DNA-directed RNA polymerases I,II,and III subunit RPABC2,RPB6 homolog

RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5

hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein

hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit

EMD-12971 was used to generate this composite map.
EMD-12972 was used to generate this composite map.
Pol II, Super elongation complex, ELL2, EAF1, TRANSCRIPTION