Structures of ABCG2 under turnover conditions reveal a key step in the drug transport mechanism.

ABCG2 was incubated with 5mM ATP, 5mM MgCl2, 0.5mM ADP, 100 uM topotecan at room temperature for 10 min
ABCG2 in complex with topotecan under turnover condition

Grids were blotted for 2.5s with blot force 1

Broad substrate specificity ATP-binding cassette transporter ABCG2

ATP-binding cassette sub-family G member 2,Breast cancer resistance protein,CDw338,Mitoxantrone resistance-associated protein,Placenta-specific ATP-binding cassette transporter,Urate exporter

ABC transporter plasma membrane ATP multidrug resistance, TRANSPORT PROTEIN

(chain "A" and (resid 34 through 654 or resid 1502))