Snapshots of the large fragment of DNA polymerase I from Thermus Aquaticus processing C5 modified thymidines

2'-deoxy-5-[9-(3-{[4-(diethylamino)-4-oxobutanoyl]amino}propyl)-18-ethyl-5,8,14,17-tetraoxo-4,9,13,18-tetraazaicos-1-yn-1-yl]uridine 5'-(tetrahydrogen triphosphate)

Structural basis for the synthesis of nucleobase modified DNA by Thermus aquaticus DNA polymerase.

vertically collimating mirror (M1, focus at infinity), followed by a Bartels Monochromator with dual channel cut crystals

Bartels Monochromator with dual channel cut crystals and a toroidal mirror (M2)

DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3')

DNA (5'-D(*AP*AP*AP*AP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3')

0.05M Na cacodylate ph 6.5, 0.2M NH4OAc, 0.01M Mg(OAc)2, 26% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K

DNA polymerase I, thermostable (E.C.2.7.7.7)/DNA complex

DNA polymerase, C5 modified nucleotide analogs, binding pocket, denron-labeled triphosphate, Transferase-DNA complex