Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants

Anatomy of Glycosynthesis: Structure and Kinetics of the Humicola Insolens Cel7B E197A and E197S Glycosynthase Mutants

HANGING DROPS 20MM TRIS-HCL PH7-8.5, 280 15-30% POLYETHYLENE GLYCOL 4000, pH 7.00

HUMICOLA INSOLENS ENDOCELLULASE EGI S37W, P39W DOUBLE-MUTANT

HUMICOLA INSOLENS ENDOCELLULASE CEL7B (EG 1) E197A MUTANT

HUMICOLA INSOLENS ENDOCELLULASE EGI NATIVE STRUCTURE

SHEET
THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

ENGINEERED RESIDUE GLU 197 SER

N-LINKED N-ACETYLGLUCOSAMINE ON RESIDUE ASN 247, PYROGLUTAMATE POST-TRANSLATIONAL MODIFICATION ON RESIDUE 1

PYROGLUTAMATE
POST-TRANSLATIONAL MODIFICATION AT RESIDUE 1,
MUTATION E197S,
MISSING LAST FOUR RESIDUES.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

HYDROLASE, CELLULOSE DEGRADATION, GLYCOSYNTHASE