Crystal structure of the cysteine-rich Gallus gallus urate oxidase in complex with the 8-azaxanthine inhibitor under reducing conditions (space group P 21 21 21)

Cysteine Enrichment Mediates Co-Option of Uricase in Reptilian Skin and Transition to Uricotelism.

His6-tagged GgUOX at approx. 22 mg/ml in 50 mM Tris-HCl pH 8.0, 10 mM NaCl, 1 mM TCEP-HCl was incubated with a 20-fold molar excess of AZA. Crystals were obtained by mixing the protein with 0.8M K/Na tartrate tetrahydrate, 0.1 M HEPES pH 7.5

Biotechnology and Biological Sciences Research Council (BBSRC)

Hydrogens have been added in their riding positions

COFACTOR-FREE OXIDASE, INHIBITOR, DIOXYGEN BINDING, CYSTEINE, OXIDOREDUCTASE

Local NCS retraints between domains: 1 2

Local NCS retraints between domains: 3 4

Local NCS retraints between domains: 5 6

Local NCS retraints between domains: 7 8

Local NCS retraints between domains: 9 10

Local NCS retraints between domains: 11 12