Crystal Structure of BphD, a C-C hydrolase from Burkholderia xenovorans LB400

Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase

2.0-2.4 M ammonium sulfate, 6-10% ethanol, 0.1M Tris-HCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (E.C.3.7.1.-)

The biological assembly is a tetramer generated from the dimer in the asymmetric unit by the operation: 0.5-x, 0.866-y, z.

BphD, alpha/beta hydrolase, PCB Degradation, Meta cleavage product hydrolase, MCP hydrolase, 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase, HYDROLASE