CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA-FUCOSIDASE IN COMPLEX WITH FUCOSE

Crystal Structure of Thermotoga Maritima {Alpha}-L-Fucosidase: Insights Into the Catalytic Mechanism and the Molecular Basis for Fucosidosis

Identification of the Catalytic Nucleophile of the Family 29 Alpha -L-Fucosidase from Thermotoga Maritima Through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis

18% PEG600, 5% JEFFAMINE M-600, 100 MM TRIS-HCL PH 8.0, PROTEIN CONCENTRATION 5 MG/ML FUCOSE WAS INTRODUCED BY SHORT SOAKING OF A NATIVE

CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA -FUCOSIDASE

CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA -FUCOSIDASE IN COMPLEX WITH A MECHANISM BASED INHIBITOR

HELIX
DETERMINATION METHOD: AUTHOR PROVIDED.

SHEET
DETERMINATION METHOD: AUTHOR PROVIDED.

ALPHA-L-FUCOSIDASE BELONGS TO GLYCOSYL HYDROLASASE FAMILY 29. THE ENZYME PERFORMS CATALYSIS WITH RETENTION OF CONFIGURATION AT THE ANOMERIC CARBON. THE COORDINATES GIVEN DEFINE THE STRUCTURE OF ALPHA-L- FUCOSIDASE IN COMPLEX WITH FUCOSE. FUCOSE HAS UNDERGONE MUTAROTATION AND IS PRESENT AS THE BETA-CONFORMER.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. SOLVENT NO WATER MOLECULES HAVE BEEN ADDED GIVEN THE MODEST RESOLUTION AND THE HIGH OVERALL DISORDER OF THE STRUCTURE.
NATIVE ALPHA-L-FUCOSIDASE, PDB ENTRY 1HL8

PUTATIVE ALPHA-L-FUCOSIDASE (E.C.3.2.1.51)

BETWEEN THE ISOLATED CHAINS AND THAT FOR
THE CHAINSIN THE COMPLEX IS IN AVERAGE 2464 (+/-
52) ANGSTROM**2.

HYDROLASE, GLYCOSIDE HYDROLASE, ALPHA-L-FUCOSIDASE, PRODUCT COMPLEX, THERMOSTABLE