Family 1 b-glucosidase from Thermotoga maritima

Iminosugar Glycosidase Inhibitors: Structural and Thermodynamic Dissection of the Binding of Isofagomine and 1-Deoxynojirimycin to Beta-Glucosidases

GENTIOBIASE, CELLOBIASE, BETA-D-GLUCOSIDE GLUCOHYDROLASE

10MG/ML PROTEIN IN 15% PEG 4K, 0.2 M CA ACETATE, 0.1M IMIDAZOLE PH 7.0

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY
A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY
A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

A1,2,305-307,446, B307,B213,B446 POOR OR MISSING DENSITY THE FULL LENGTH PROTEIN ALSO CONTAINS A HIS-TAG FROM THE PET28A CLONING VECTOR. NUMBERING OF THE PROTEIN AGREES WITH THE SWISSPROT ENTRY AND THUS IGNORES THE HIS-TAG

HYDROLASE, GLUCOSIDE HYDROLYSIS, FAMILY GH1, ENZYME, GLYCOSIDASE, CELLULOSE DEGRADATION