Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the Apo form

The structure of flavin-dependent tryptophan 7-halogenase RebH.

Adjustable focusing mirrors in K-B geometry

Putative halogenase of tryptophan, Halogenase

Protein Solution (18 mg/ml protein, 0.050 M sodium chloride, 0.010 M TRIS pH 8.0)
mixed in a 1:1 ratio with the Well Solution
(0.9 M K2HPO4, 0.5 M NaH2PO4)
Cryoprotected with: well solution supplemented with up to 30% glycerol, vapor diffusion, hanging drop, temperature 277K

L-TRYPTOPHAN/FAD COMPLEX OF SAME PROTEIN

Solvent flattening and Histogram matching

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

RebH, Rebeccamycin, halogenase, flavin, BIOSYNTHETIC PROTEIN, FLAVOPROTEIN