Crystal structure of wild type Aplysia californica AChBP in complex with nicotine

(S)-(-)-NICOTINE; 3-[(2S)-1-METHYL-2-PYRROLIDINYL] PYRIDINE

Engineering a surrogate human heteromeric alpha / beta glycine receptor orthosteric site exploiting the structural homology and stability of acetylcholine-binding protein.

0.45 M ammonium phosphate monobasic
2 % glycerol
2 % IPA

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS