Crystal structure of the peptidase domain of collagenase H from Clostridium histolyticum in complex with N-aryl mercaptoacetamide-based inhibitor

Discovery of a Potent Inhibitor Class with High Selectivity toward Clostridial Collagenases.

Non-aligned residues at N-terminus were not ordered in crystal. GT = position 1-2, remainder of tag after cleavage LDK ... PNEG = position 3 -394, sequence of peptidase domain of ColH, corresponding to L331-G721 of Uniprot entry Q46085

polyethylene glycol methyl ether 2000, NaCl, MES

contains the same protein comlexed with ISOAMYLPHOSPHONYL-GLY-PRO-ALA

HYDROLASE-INHIBITOR COMPLEX, GLUZINCIN, METALLOPROTEASE, collagenase, HYDROLASE