Structure of the E100A E.coli GDP-mannose hydrolase (yffh) in complex with GDP-mannose and Mg++

Structural studies of the Nudix GDP-mannose hydrolase from E. coli reveals a new motif for mannose recognition.

20-26% PEG 3350, 0.2 M Mg Cl, 0.1 M Tris HCL pH 8.5, 4mM GDP-mannose at a ratio of 1:1 protein:reservoir, vapor diffusion, hanging drop, temperature 291K

E. coli GDPMK E100A mutant in complex with Mg++

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

GDP-mannose pyrophosphatase nudK (E.C.3.6.1.-)