Effects of alpha-tubulin acetylation on microtubule structure and stability.

We use PHENIX to perform real space refinement and sharpen our cryoEM maps.

Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).

Preliminary grid screening was performed manually and all of the alignments were initially done using a gold calibration grid.

Acetylated and deacetylated tubulin preparations were produced by treating purified brain tubulin with the acetyltransferase TAT1 or the tubulin deacetylatase SIRT2 as done in Portran et al. Nat. Cell. Bio. 2017.

Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain

National Science Foundation (NSF, United States)

Tubulin alpha-1B chain, Tubulin beta chain
microtubule, cytoskeleton, acetylation, STRUCTURAL PROTEIN