Human phosphomannomutase 2 (PMM2) wild-type in apo state

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Insight on molecular pathogenesis and pharmacochaperoning potential in phosphomannomutase 2 deficiency, provided by novel human phosphomannomutase 2 structures.

Crystals of hexagonal morphology and 0.025 mm in the largest dimension.

Drops made by mixing 1 microliter of protein solution and 1 microliter of crystallization depoit solution. Protein solution contained 5 mg/ml protein in 20 mM Hepes pH 7.5 and 0.2 M NaCl. Crystallization solution contained 0.3-0.4 M MgCl2, 24% PEG3350 and 0.1 M HEPES pH 7.5

Spanish Ministry of Science, Innovation, and Universities

Spanish Ministry of Economy and Competitiveness

Incomplete model without cofactors and missing loops

glycobiology, congenital disorders of glycosylation, phosphotransferase, phosphomutase, ISOMERASE