ECAII(D90T,K162T) MUTANT IN COMPLEX WITH CITRATE AT PH 5.6

Opportunistic complexes of E. coli L-asparaginases with citrate anions.

Crystal structure and allosteric regulation of the cytoplasmic Escherichia coli L-asparaginase I.

L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II

ORF contains a secretion sequence, 'HHHHHH' affinity tag and sequence of doubly mutated mature EcAII

Protein, at the concentration 15 mg/ml in 50 mM HEPES buffer pH 7 and 150 mM sodium chloride was mixed with equivolume solution of precipitant that contained, 17% (w/v) PEG3350, 0.17 M ammonium citrate pH 5.6, and 20 mM L-Asn. Resulting droplets were equilibrated against the precipitant. For the data collection, crystal was mounted in a quartz capillary

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

inactive mutant, hydrolysis of L-asparagine, HYDROLASE