Structural Evidence for a Common Intermediate in Small G Protein-GEF Reactions

Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana

residues 76-440 based on the database numbering

18%(v/v) Tacsimate(Hampton Research), 4%(w/v) PEG 3350, 0.1M Tris-HCl, pH 7.3, vapor diffusion, hanging drop, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

complex of PRONE-GEF with Rop substrate, SIGNALING PROTEIN