Isatin hydrolase A (IHA) from Labrenzia aggregata with isatinate bound

A fundamental catalytic difference between zinc and manganese dependent enzymes revealed in a bacterial isatin hydrolase.

15 mg/mL in 5 mM tris-HCl 7.5, 100 mM NaCl 1.0 mM MnCl2, 20 % glycerol and 20 % PEG 1500

Isatin, hydrolase, Labrenzia aggregata, isatinate