Cytoplasmic domain of the Thermomonospora curvata Type VII Secretion ATPase EccC

Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion.

Cytoplasmic domain of EccC ATPase, UNP residues 200-1315

EccCTc(199-1315) was concentrated to 14 mg/ml and adjusted 10 mM ATP from a 100 mM stock (Roche). The protein was mixed with a 1:1 volume of 0.1 M Tris pH 8.0, 0.6-1.1 M (NH4)2SO4, 0.1 M glycine, 0.020 mM MgCl2, 0.1 M NaCl,, equilibrated in a vapor diffusion experiment at 20 C over 500 mL of the mother liquor., VAPOR DIFFUSION, HANGING DROP, temperature 298K