Crystal structure of FnCas12a bound to a crRNA

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.

SNA sequence at the N-terminus is derived from expression vector.

Francisella tularensis subsp. novicida (strain U112)

50 mM HEPES-NaOH, pH 6.5, 20% (w/v) Polyethylene glycol 3,400, 1.5% (w/v) Tryptone

chain 'A' and (resid 1037 through 1299 )
chain 'C' and (resid 1037 through 1299 )
chain 'G' and (resid 1000 through 1299 )
CRISPR-associated endonuclease Cpf1/RNA Complex
CRISPR, Cas, Cpf1, Cas12a, nuclease, genome editing, R-loop, crRNA, RuvC, hydrolase