Crystal Structure of Glutamate Carboxypeptidase II in a complex with urea-based inhibitor

N-{[(1S)-1-carboxy-5-({[2-({4,44-dioxo-48-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-7,10,13,16,19,22,25,28,31,34,37,40-dodecaoxa-3,43-diazaoctatetracont-1-yl}oxy)phenyl]carbamoyl}amino)pentyl]carbamoyl}-L-glutamic acid

Rational design of urea-based glutamate carboxypeptidase II (GCPII) inhibitors as versatile tools for specific drug targeting and delivery.

Extracellular domain, UNP residues 44-750

Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase

Tris-HCl, PEG 3350, pentaerythritol propoxylate, pH 8.0, vapor diffusion, hanging drop, temperature 289K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES: REFINED INDIVIDUALLY

Glutamate carboxypeptidase 2 (E.C.3.4.17.21)

hydrolase, glutamate carboxypeptidase II, PSMA, NAALADASE, urea-based inhibitor, caboxypeptidase, glycoprotein, metallopeptidase, HYDROLASE-HYDROLASE INHIBITOR complex