Crystal structure of the double mutant (Cys211Ser/Cys292Ser) 6-phospho-b-D-glucosidase from Lactobacillus plantarum

Structural basis of the substrate specificity and instability in solution of a glycosidase from Lactobacillus plantarum.

20% (w/v) PEG 3350, 0.2 M sodium citrate and 50 mM spermidine

Analytical gel filtration and ultracentrifugation reveal the presence of a large oligomer (hexamer).
cysteine residue, glycosides, protein aggregation, reducing agent, solubility enhancer, hydrolase