Crystal Structure of a Pantothenate Synthetase from M. tuberculosis in complex with AMPCPP

TB Structural Genomics Consortium (TBSGC)

DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER

Crystal structures of a pantothenate
synthetase from M. tuberculosis and its
complexes with substrates and a
reaction intermediate

Pantoate--beta-alanine ligase, Pantoate activating enzyme

PEG3000, lithium sulfate, magnesium sulfate, imidazole, ethanol, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

apo enzyme structure of M. tuberculosis pantothenate synthetase

CRYSTAL STRUCTURE OF A PANTOTHENATE SYNTHETASE FROM M. TUBERCULOSIS IN COMPLEX WITH AMPCPP AND PANTOATE (BETTER OCCUPANCY OF PANTOATE MOLECULE THAN 1N2E)

CRYSTAL STRUCTURE OF A PANTOTHENATE SYNTHETASE FROM M. TUBERCULOSIS IN COMPLEX WITH AMPCPP AND PANTOATE

CRYSTAL STRUCTURE OF A PANTOTHENATE SYNTHETASE FROM M. TUBERCULOSIS IN COMPLEX WITH A REACTION INTERMEDIATE, PANTOYL ADENYLATE

CRYSTAL STRUCTURE OF PANTOTHENATE SYNTHETASE FROM M. TUBERCULOSIS IN COMPLEX WITH A REACTION INTERMEDIATE, PANTOYL ADENYLATE, DIFFERENT OCCUPANCIES OF PANTOYL ADENYLATE.

crystal structure of a pantothenate synthetase from M. tuberculosis in complex with pantoate

CRYSTAL STRUCTURE OF PANTOTHENATE SYNTHETASE FROM M. TUBERCULOSIS, LOW OCCUPANCY OF BETA-ALANINE AT THE PANTOATE BINDING SITES

SEQUENCE
RESIDUE 2 IS AN ALANINE IN THE SEQUENCE.
MUTATION OF THIS RESIDUE WAS NECESSARY TO
GENERATE AN NCOI RESTRICTION SITE
FOR CLONING INTO THE EXPRESSION VECTOR PET30A.

THE LAST 9 RESIDUES AFTER ARG300 WERE CLEAVED
OFF BY ENTEROKINASE DIGESTION (UNINTENTIONALLY),
WHICH WAS CARRIED OUT TO CLEAVE THE N-TERMINAL
TAG FROM THE RECOMBINANT PROTEIN.

Structural genomics, Rossmann fold, dimer, intersubunit beta sheet, PSI, Protein Structure Initiative, TB Structural Genomics Consortium, TBSGC, LIGASE