CRYSTAL STRUCTURE OF FATTY ACID AMIDE HYDROLASE

Structural Adaptations in a Membrane Enzyme That Terminates Endocannabinoid Signaling

Oleamide hydrolase, Anandamide amidohydrolase

PEG 6000, 2,4-methyl pentanediol, sodium citrate, lithium sulphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Refinemant carried out while the manuscript was in press resulted in improved geometry
at the cost of slightly higher R values. Atoms for which no density was observed were
not included in the refinement.
Refinement was performed by first detwinning the data using the molecular model.
Followed by rounds of refinement and rebuilding and additional iterations of the detwinning procedure.
No sigma cutoff was used due to details of the detwinning.
The final Rtwin = 0.272

FAAH structure in an alternate spacegroup. Solved by MAD/MIRAS phasing.
Fatty-acid amide hydrolase (E.C.3.1.-.-)